Coding

Part:BBa_K2022010:Design

Designed by: Jessica Mazalo, Daniel Winter   Group: iGEM16_UNSW_Australia   (2016-10-11)


RFP, with Periplasm-localisation Tag


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 54
    Illegal AgeI site found at 621
    Illegal AgeI site found at 733
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Translation begins at the Met of the signal tag, before continuing into the RFP protein. We ultimately decided to keep the Met that begins RFP, as we couldn't find any evidence to suggest it is cleaved off in the mature, fully-processed form.


Source

The sequence for RFP was the open reading frame of BBa_J04450. To its n-terminus we affixed a 20AA sec-signal tag, which guides the fusion protein to the periplasm.

References